LOCUS BG072740 794 bp mRNA linear EST 12-MAY-2010 DEFINITION H3114D08-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3114D08 3', mRNA sequence. ACCESSION BG072740 VERSION BG072740.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 794) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG072740.1. Other_ESTs: H3114D08-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3114 row: D column: 08 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..794 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3114D08-3" /db_xref="taxon:10090" /clone="H3114D08" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 179 a 202 c 189 g 224 t ORIGIN 1 ctcaaaaatg ttttaattca atgtctaaca taagcagtga catttattat atattaagta 61 ccagcttaaa actgccaaaa atcgaaatca cccgacaccc accctcccaa ttcttgacca 121 agagcttttc cagcaaagac aggctctctt ctctccttga tacggtaggt atgaacagac 181 acatcggcct gtgtgtccta tgatgtgtct ccacgtgatg gtgctgccca ggctcctctc 241 actgcccagg ccccgaaaga ctcttgttca tgaactgtct cttcacaaag caagtccacc 301 acttgctggg tttatcactc tgagggtcaa aaaccttctc acacagcctc agtccggtcc 361 cttgcctcag ctgctggagg tacgcccgca tcatctcgtc ctcctgtttg ctcgcaggct 421 tggcataaat ggcgttgaga ggaaagccag gctctccggg gatagggaag ttggtgattc 481 ctagcgtgta catttccttc tcgccttggc tcttggagtt gcacttttgg agtttcttta 541 gacactcaga aatgtagagt gtgatgtaga tcaatgtcct gtccgcttca ttcttaattt 601 catagttctt gaagaagaca ttggccttga agtagtagat ggcttcatcc acaatgtccg 661 tgtctttggt ctcgcgaggg gcgggtcctt tgaactggct tcggaggggc aacagggcca 721 tgttaccgat gagcttggtg tccgggtcca tgagagaaga gtggtatgcc ggcatcttga 781 cggtgtccgg attc //