LOCUS BG072708 620 bp mRNA linear EST 12-MAY-2010
DEFINITION H3114A07-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3114A07 3', mRNA sequence.
ACCESSION BG072708
VERSION BG072708.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 620)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG072708.1.
Other_ESTs: H3114A07-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3114 row: A column: 07
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..620
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3114A07-3"
/db_xref="taxon:10090"
/clone="H3114A07"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 246 a 93 c 116 g 165 t
ORIGIN
1 aaacaacact ataattataa agttgaaggc acatcttcgg cagaaatatc aattacatgc
61 acatgagaaa aaccaaacta aactacagaa acgaaatatt gcacaaagtt gcccgctcct
121 gttttggatt ctaaccaaaa tttcataata catttcttta aaaacacaag gttgagacac
181 ctggctctgg ctacaaaagt caattattat caacatcatt aaagaagcta atgtgattga
241 gaaataagaa ttcatgcttt gtgttatagc tagctactca taccagacga aaggacttaa
301 gaaagttagt gtaaaagttg gtaaagattt ggcatgaatc aatctatcta tctatctatc
361 tatctatcta tctatctatc tatctatcta tctatctatc tatctatcta tctatcgata
421 tttggtaaag aagtatgttt tttaatacag aaagcaaaat aactcaaagg agtgattatg
481 aggaatgtct gtataaaaag gagaaggaag aagagaagga agagggagag gaggaggagg
541 aagaggcgga ggaagaggaa gaagagaagc agcttaagct ttaaccagat gcctcataca
601 aaaaaataac aaagtagagt
//