LOCUS BG072657 501 bp mRNA linear EST 26-JAN-2001 DEFINITION H3113D09-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3113D09 3', mRNA sequence. ACCESSION BG072657 VERSION BG072657.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 501) AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A., Tanaka,T.S., Carter,M.G. and Ko,M.S.H. TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set JOURNAL Unpublished COMMENT Other_ESTs: H3113D09-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3113 row: D column: 09 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..501 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3113D09-3" /db_xref="taxon:10090" /clone="H3113D09" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 175 a 93 c 93 g 140 t ORIGIN 1 acattttatt cacatttatt tttttgcttt tagggggctc acagaaaatt agaacacctt 61 aagcaggagt tgaatagcaa tgcttggaag caacgttaca ttccagcttt atggcaaatt 121 ggccaaagct cctccagtat ttacaaaaca cgagagacga gatgctacac aaaaccattg 181 catctgaaga tctgtttctc ctttattctc aaagacaact ggaaaggaaa gcattgtctg 241 ctgtaatcaa aaaacatacc acagtataaa cagtaaccat tccacgtatc acaggttggt 301 tgagtttaaa attttgggtt taaaagggcc aagatgactg cagttttaca aaaatgggca 361 ggggggagac ttgcaaactt catgggcttc tgaaggatat caagatttgt ttttatacaa 421 tagtcacagt taaaaacacc ctgctggtaa tacataatta cactttatta agggcataaa 481 ccagcaataa acaataaagc c //