LOCUS BG072568 648 bp mRNA linear EST 12-MAY-2010 DEFINITION H3112D07-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3112D07 3', mRNA sequence. ACCESSION BG072568 VERSION BG072568.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 648) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG072568.1. Other_ESTs: H3112D07-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3112 row: D column: 07 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..648 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3112D07-3" /db_xref="taxon:10090" /clone="H3112D07" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 203 a 149 c 146 g 150 t ORIGIN 1 atgttaagca agtttattaa aaataacgct aatgtgatcc cagggcacac taatttcaag 61 agggtctttg caacagattt ttatcataac cacttatcaa ctaaacaaca atgaacttaa 121 cttatagaat caaataacag aggaaaagga ccaggctaat cctgtaggga ctgagctgcc 181 cttgatcttc cttaaaccga ccagcctgca gcccttggca aagctggtct ctagagggtt 241 aatcaggacc agaactgagc aatgagctcc tactgcacct gctaaggctc ttaaaagtca 301 ggagccagaa agaaagcagg aaaggtgtcc tacactggtc cagctgttac aaatgattaa 361 aggcctgtta aagatttcca tgttcactat ttcaaaatgc tagttttaaa aaacgtcaca 421 ggcaacgcac gtatttcccc tccccatctc ttaaacccga acacccagcg tagaaaccct 481 ctcgggagaa ccagagagag aggaagaggg gaacagatgg tgtgtggcag atgctggtac 541 tgctgagggg ctgtggatgc cagctgtggc aacactaaat cccccaataa ggcatcaagt 601 gtcgggactc cttctcataa acgtctggga tgatgccata gggtgtct //