LOCUS BG072448 697 bp mRNA linear EST 12-MAY-2010 DEFINITION H3110F05-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3110F05 3', mRNA sequence. ACCESSION BG072448 VERSION BG072448.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 697) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG072448.1. Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3110 row: F column: 05 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..697 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3110F05-3" /db_xref="taxon:10090" /clone="H3110F05" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 215 a 166 c 119 g 197 t ORIGIN 1 aatcttccta atgaaaatct tatcttcaag gtgagaattc tggatctaaa atacctaata 61 aggataccaa gcagaataat ctgccaagat aaaaacatcc tccactgctt ttgttgtttt 121 ccctctctta tttatatgca gatttggaag ccacctggtt tacaggatgt gtcttttagg 181 cttaaaagcg taagtgaaac aaaaatatct aatgcatgct gtcagccaaa gcccatgaga 241 agtcatcgag acttgagaat catcagcgca tgggacttga ttctccggga gtatagaaca 301 aaatagcctc caatgtagct acgacccttg cagtttctca acactgagca aaaagatgct 361 tcattaacat gaaagtgttt tctcaaaggc aatcacgtgc agttacctga gcctcagttt 421 cttattagta caggcccacc gaagcccctt tccgccctac acccatataa tggctgtgga 481 aatgacaaag acggtctact gcctggcacc agttccaaac tgatccaata aatgtgtaac 541 atcaaagcca tatcagtatc cactccagaa acgcacagcc tccgtccagc cttccgctca 601 ctgtgcccca caaattataa tggaaaggtc cctcctaacc ctaagtcttt taaaaatttg 661 ttattatttt ttaaattagt atctgatttg attcctg //