LOCUS BG072427 836 bp mRNA linear EST 12-MAY-2010
DEFINITION H3110D06-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3110D06 3', mRNA sequence.
ACCESSION BG072427
VERSION BG072427.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 836)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG072427.1.
Other_ESTs: H3110D06-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3110 row: D column: 06
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..836
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3110D06-3"
/db_xref="taxon:10090"
/clone="H3110D06"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 222 a 192 c 181 g 241 t
ORIGIN
1 aaaatatttc tcagacatag acttttgagc ctaggacagg caggacagct gagttccaga
61 cttctgagac tcaccatatc ctcactttta gcttcttctg ggattatttt tctagctgta
121 ctctgcagtc cctagagcaa acagcacctg ccttaggtct cccactgaac cagtactttg
181 tttttataat gtcctgggtt tcactagtgg attaagtgaa aaaagatgat tagtgccaga
241 tttctatttt cagtggccca tgggctatag ccattagcaa cttggtcctt gtggccccta
301 gtcatcttaa agcaatactc cactcctcaa ttctccttgg gcaaatgagc agatttcaca
361 gggcagagtt tttctcctgg gctccttatt ttcactccaa gtgtgcacag gcaccatggc
421 cagaattaga gaaggcatgt gttctacaat aagactccaa gagtaagggt tttgtcttct
481 tttgcaatta cgtgacatac atttgtgtat gggtatgagg atatgaatgc agatatgagt
541 gcccacagag accagaggca tcaggcatcc ctagacctgg agttgcaagt ggttgtaagt
601 tgcctgatat agggctggga actaaattca ggtcctataa aagcagtatg tactcctaag
661 caccaagcaa tctctttagt ctctgtcttc ttttgactgt acgtaaaagc ctaagacagg
721 taacagatca aggaaaggct gctatggttg agcaaacatc cagagaccct caaacacagg
781 actttccgac tttagcttac ccaggccgct cgctgctcct gctacttgag tggaca
//