LOCUS BG072409 677 bp mRNA linear EST 12-MAY-2010
DEFINITION H3110B11-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3110B11 3', mRNA sequence.
ACCESSION BG072409
VERSION BG072409.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 677)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG072409.1.
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3110 row: B column: 11
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..677
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3110B11-3"
/db_xref="taxon:10090"
/clone="H3110B11"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 239 a 123 c 105 g 210 t
ORIGIN
1 cttagatgtc tgtattttat ttagattcta tcacataaac atacacagct tctgagacta
61 atacattctt taactgtacc agctttaaga cttctttcta tcttctgttt gtaattctca
121 gaattaagga tcttttcaaa agctttaagg tacaagtcaa aacaaaaagt tccaaagaaa
181 aggtcatttt aataataaag ctacatttca ccacaagaaa tgaaacattt aaatatagca
241 tatggcatac atggagttat acattctagt gtcattttcc ttgttcagac ttttgaatgt
301 ttaaagtcta ttaaagtgac atactgggac caacttgggg ccccctttca aagcactgac
361 tctttttttt tattacagtt agtcaaatgc aggagcaaac aaaagaaaat tccaagatct
421 aatagaaaaa caacagcaaa accatgaaaa tagccacctg tatgttactt gattcattca
481 gctgaaaagc cattcaatgg tttggagcaa caatttggcg attttaaagg ggtgacatga
541 aagggtcctt gctagattca gctcccataa gggaaaagaa ataattgcca ctatttactt
601 attcattcaa ccaaaccttt cttcaacccc taaacagtaa aggagagtaa tgctctgcag
661 atgatggagc ttaataa
//