LOCUS BG072348 567 bp mRNA linear EST 12-MAY-2010
DEFINITION H3109E05-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3109E05 3', mRNA sequence.
ACCESSION BG072348
VERSION BG072348.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 567)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG072348.1.
Other_ESTs: H3109E05-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3109 row: E column: 05
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..567
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3109E05-3"
/db_xref="taxon:10090"
/clone="H3109E05"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 140 a 150 c 136 g 141 t
ORIGIN
1 ctcagaaaga aaaaccgaga tggggaacta gtggtgggat gttcctctaa tcccagaact
61 ctcaatttgg gaggttgagg caggaagaat gtcagttcaa ggccaacttg ggctataagg
121 tgagacccta tcaaaacaaa agaaaagaaa gaaaagacaa aaaccttcag tcctaatgtt
181 tagcttcagt cctgcactta agaagaatcc actgtaatgg ggttagcctt gtcaagcatc
241 gtctcttgtc aagcatcgcc caccagatca gggacggagt ggggatatct ctcagttgtt
301 tcttgaattg ttccagcagc cagagccctc cctttccccc ggctcctccc tgctctcttg
361 ttcaaagtag gcgtcttctt cttcttcttc ctcacgtatc accttcatca gctgcaaaaa
421 ggtggggagt gggccctgat ctttcagctc ctgtagcctg caccacagaa cgttgccgag
481 gttggcacca gccatgacct gctccaggcg tacctggtca gcaatgttcc gggggatggc
541 ccttttttcc acagctctcc ggagcag
//