LOCUS BG072289 587 bp mRNA linear EST 12-MAY-2010 DEFINITION H3108H05-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3108H05 3', mRNA sequence. ACCESSION BG072289 VERSION BG072289.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 587) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG072289.1. Other_ESTs: H3108H05-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3108 row: H column: 05 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..587 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3108H05-3" /db_xref="taxon:10090" /clone="H3108H05" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 161 a 150 c 136 g 140 t ORIGIN 1 cttatttatt ttgtgtgagt tcacatgcca cggtgcacac atggaggtcg gaggtcaact 61 ttcaactcaa gcctttcatc acgggggtcc cctggatgga actcagggtt ttgagactgg 121 cagcctttac ccactgagtc attttgctag ccccatctaa actcaacaaa atgtctacct 181 gcaaagccaa gggggccaaa cctagtgtgt ggatgataca gtggaaacaa gagccttgcc 241 gacgatggct gaagtgctga caagggcagg aaattaaaac tgcccaaaaa gaaccacatg 301 tgtttaggga cacatttcct gaagttcaat gccacagcca cctgacagag gagaaaactt 361 atcctctaaa ccggattcgt gcggattcgt tttcaaacac cagtgaccca tgaagactat 421 ggccagatga tttttagctt ttacctctca cactaaatat ggtccccgag gcggagtgac 481 ggcaattcaa caggcagggg cgcgtggccc atctgataag cctccctcct cccttccaag 541 atgcagagag aaagccttac tttcacttct ctagatctag gctgaac //