LOCUS BG072249 675 bp mRNA linear EST 26-JAN-2001
DEFINITION H3108D10-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3108D10 3', mRNA sequence.
ACCESSION BG072249
VERSION BG072249.1
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 675)
AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A.,
Tanaka,T.S., Carter,M.G. and Ko,M.S.H.
TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set
JOURNAL Unpublished
COMMENT Other_ESTs: H3108D10-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3108 row: D column: 10
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..675
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3108D10-3"
/db_xref="taxon:10090"
/clone="H3108D10"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 146 a 180 c 139 g 208 t
ORIGIN
1 aattacgttt tctctttcac aactgtgtaa tgtatgcaat cattttgatc agcttcttat
61 gtcctctgat cctccctctt atgtcccctg ccctcctgcc ctctcaccca cctcctcaca
121 ggtctcattt ccacatgcat gtcttttgtt ttgtgactca ctgggttgag agacatttat
181 gtgatcacgg gtttggaact atctactgga gtgtggtggg ctcaccagta gaaacaagac
241 tgaaggcaat gactgcccct ttcccagaat ccacctgggc caacaggtca gctgggaagg
301 agtagggttc caaaagcccc ctccttcatt atgagagatg gttacanttt gggaggcccc
361 agtctcatgc aggtccagtc caggcaactg tagctgctgg gaggtcctga tcgcactgct
421 aggtctagcg cggaagtgtg ttctccagct cttctcctca tcttccagcc ctcacttctt
481 gtcccctctt ctgtgatgtt tcctgagcct taaaagggtt catgccagtt tagagctgag
541 ctcccaacca tcacttattc ttggtatctt tatgtagctg taactctctg gatttactgc
601 cactaccaag agaagttcct ttgataaagg ctgagcatag catttgtcta cagctnataa
661 cataaatatt ttaca
//