LOCUS BG072230 727 bp mRNA linear EST 12-MAY-2010
DEFINITION H3108C01-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3108C01 3', mRNA sequence.
ACCESSION BG072230
VERSION BG072230.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 727)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG072230.1.
Other_ESTs: H3108C01-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3108 row: C column: 01
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..727
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3108C01-3"
/db_xref="taxon:10090"
/clone="H3108C01"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 194 a 149 c 187 g 197 t
ORIGIN
1 gacctttttg tttttttatt ggggtgtggg ggtagacagg gtcacggtat atagcccagg
61 ataaccctga actcctggtt cctcctgtct cccaggtgct gggattacag gtgggtacca
121 ttattcctgg ctccacagtg ttttaaagtg ttcaggcatg caagcccaga gcagactttg
181 tactttccgg tacaggggga cactggatag aaatattcac acagaaactc caggtcagca
241 ggaagttcaa agcaccattc aaggcaggta agaaagttcc ttcaacttgc tgttccaaaa
301 atctctagac agccacaatc tcaagagcat gctatcttgg tgcactttgg tttcttaaaa
361 caaaaacaaa acacaggtaa gaatgttttt gttttaatct caggtgtggg atgccaggct
421 gcttcagact gtccacagca gctgactatg atttgcctca tgctctagca ggagtgtagt
481 tttgccagca gcagacagtt tctctgagtg tgtgatgatt ggaatttctg gggacttttc
541 agaagtacat aagtgctagg gcccccgaga agcggggtgg gttgttgttt gctgttggtc
601 ggtgtttgtg aagtggtcgt gggcgaagaa gaaacaagaa gaaatgagat atcttggcag
661 caaagatcat tgctccaagg aactcgacac cccttaatca gcaagaagtc gtccaacaat
721 ataacag
//