LOCUS BG072151 643 bp mRNA linear EST 12-MAY-2010
DEFINITION H3107C12-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3107C12 3', mRNA sequence.
ACCESSION BG072151
VERSION BG072151.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 643)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG072151.1.
Other_ESTs: H3107C12-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3107 row: C column: 12
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..643
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3107C12-3"
/db_xref="taxon:10090"
/clone="H3107C12"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 193 a 106 c 112 g 232 t
ORIGIN
1 gagacagggt ttctctgtat tgccctggct gtcctggaac tcactctgta aaccaagctg
61 gcctcgaact cagaaatccg cctgcctctg cctcctgagt gctgggatta aaggcgagtg
121 ccaccatgcc cggcttatat gtgaaattct tatggagtag aaatattgtg ggctgtaact
181 atgcggtact caatcttaca ttatggctat agttaacttt accttagaga cagtttttgg
241 ttctgtggtg tttggttctt taaaatttat ttatagggaa ttctgtagta tttaaagtag
301 aactgtcttt ttcacctact agaatttact atttgtatgt aatttgagtg aaatttgttt
361 gcaaaagctt ccaaatgtta ttcagtcaaa atttatttag atcatgtctt agaagtcaac
421 atgtagaagt caaagaacaa ctctcatgtt agggtttagt agctgaagat tattagcatt
481 aaaaataaat attaataaat ttcagtctgt ctactcaaca agaaagaaat aacttcaatg
541 tttccaacaa aatcaatcaa ctctactatg tacaaatctg ggacattaaa agagagcttt
601 ttttttttct ttctcttttt ttaaaaagat ttatctattt att
//