LOCUS BG071999 665 bp mRNA linear EST 12-MAY-2010 DEFINITION H3105E10-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3105E10 3', mRNA sequence. ACCESSION BG071999 VERSION BG071999.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 665) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG071999.1. Other_ESTs: H3105E10-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3105 row: E column: 10 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..665 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3105E10-3" /db_xref="taxon:10090" /clone="H3105E10" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 170 a 143 c 161 g 191 t ORIGIN 1 gaagtaaatt caacttttat tcttctttac aatacatgga tatgtggata aatttttctt 61 ttaagagctt gcaaccctga ggcaatgtct gtgggtacat aatgtataat gcaacagcga 121 atggagtctg aatgtgcagg gacaatggac ttagaaacat aattgaacat cgtgacgttg 181 cacacagcta tgctttgtct agtctcttaa cccaactatc tcaactgtct cgtggtctgc 241 acactgggca tcctgcctct ccctatgctt tgtcatgtat aatggcttcc ttctgacgga 301 aaagtaataa gttaacagga tttatatcta gggggtcttt atctggggtg tgtattgcca 361 gaggtgtgcc caattttgga ccacataaaa actttggccc ccaaagaaat ctggctgcca 421 tctgactgtg gtaaacgtga ggtttcttag ggtcccctgg ggtccaccac aggacaattt 481 ttcatgaggg tatggcacac tagacagaga cagaacatca ttcctgctgg ctttggacta 541 gtgggtttat tgccccatct ttcaggatgc atggcccatc ttgaggaagc agggcaacag 601 cggggtctgc aggctgacta tggttctcag ggccgagggc cggaacagag accatcacac 661 tgttt //