LOCUS BG071948 489 bp mRNA linear EST 12-MAY-2010 DEFINITION H3105A05-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3105A05 3', mRNA sequence. ACCESSION BG071948 VERSION BG071948.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 489) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG071948.1. Other_ESTs: H3105A05-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3105 row: A column: 05 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..489 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3105A05-3" /db_xref="taxon:10090" /clone="H3105A05" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 112 a 116 c 136 g 125 t ORIGIN 1 cttttctttt cttttctttt tttttccttt tttacaattt tatttataac aatatctgtg 61 ttatttagtt gtaaaggaat tcagcaaaaa ttattaaaac gttcacgtcc ccaaaatggg 121 gctgcccgct tgcccttcct tggtgtgccc cgactcttcc tggccctcag ggggatggga 181 ggcttggggg acagttacca aagaatgtgt tcagccctag gagccacaga gggggcgcta 241 gagaggggag ggcctgtctg gaagggatac tgagcatggc catctgagag gtgcccctga 301 aagtgggagc tggggtttgg ggtgacttcc ctcaactaaa agactccttc ctcagctaga 361 gccaaatctt ccagacaggg tcagcagagg actccacctc ccctgaacta accccagaga 421 gccaaggaaa ggaaagctgg gacacggagc cccgttgagt gtcttgcaca ggcgtggtgg 481 ggaggccgt //