LOCUS BG071906 803 bp mRNA linear EST 26-JAN-2001
DEFINITION H3104E04-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3104E04 3', mRNA sequence.
ACCESSION BG071906
VERSION BG071906.1
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 803)
AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A.,
Tanaka,T.S., Carter,M.G. and Ko,M.S.H.
TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set
JOURNAL Unpublished
COMMENT Other_ESTs: H3104E04-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3104 row: E column: 04
Seq primer: -21M13 Forward
POLYA=No.
FEATURES Location/Qualifiers
source 1..803
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3104E04-3"
/db_xref="taxon:10090"
/clone="H3104E04"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 197 a 132 c 147 g 327 t
ORIGIN
1 gctttttttc cactggatgg ttttagctcc cttgtaaatg atgaattgac aataggtgtg
61 ttggttcatt tctgggtctt caattctatt tcattggtct acttgtcggt cactatatca
121 gtaccatgca gtttttatca caattgctct gtagtacagc tttggtcagg catggtgatt
181 gcaccagagg ttcttttatc catgagaaga gtttttctat cctaagtttt gtttattccc
241 aatgtatttg cagattgccc tttctaattc attgaagaat tgagttggaa ttttaatggg
301 gattttattg aatctgtaga tttcttttgg caagatggcc atttttacaa tgttgatact
361 gccaatccat gataatggaa gatctttcca tcttctgaga tcttctttaa ttattttctt
421 cagagacttg aaattcttat catacagaac tttcacttac tttgttagag ttacgccaaa
481 ttattttata ttatttgtga ctattgaaaa gggagatgtt tctctaattt tattctcagc
541 ctgtttattc tttgtgaaga gaaaggccat tgacatgttt ggttaatttt atacccagct
601 aaatcaccaa cgttgttttt caggtttagg ttttctctgg tggagttttt aagttcactt
661 atatatgcta tcatatcatc tgccaaaagt ggtattttga attcttcttt tccaattttt
721 atccccttgg tctcctttca ttggcaaatt gctctctcta ggacttcaag aacaatgttg
781 aataggcagg gagaaagtgg gga
//