LOCUS BG071868 579 bp mRNA linear EST 12-MAY-2010 DEFINITION H3104A10-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3104A10 3', mRNA sequence. ACCESSION BG071868 VERSION BG071868.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 579) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG071868.1. Other_ESTs: H3104A10-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3104 row: A column: 10 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..579 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3104A10-3" /db_xref="taxon:10090" /clone="H3104A10" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 146 a 120 c 144 g 169 t ORIGIN 1 gagaactggt ttagtttatt gcagggaaat ggctatttaa acctttgtgg gggatgagta 61 ggggctttag ggagaatgta catcattggc caggtgctgg ggatgcctca tttgcataag 121 ggagcattcc aggtgctgct gaatatggcc taccacccac atctgagctc tctgccactt 181 gtggtcttgc ttctgagata gcacgcctac gcagtctgga aaagaatggt agaagcatac 241 tctaagacga actggaggag ctacctacct gttatttggg aaatgtggtg atgaaaatta 301 gaatggcccc caccagctcg cttgtttcac tattccaaac ttagtagaac tgttttgaga 361 aagataagta gatacggtct tgttggaaga agtgtgtcac tgtgaacagg ctttgaggtc 421 tcaaaagact cacaccatct acagtgtgtc tgtctgtctt tctgtctcgt gcatgtggac 481 taagacacaa gctcttagtt tgttccttca ctccgccatc atgggctctc agcacctgaa 541 actgtaagct aagttaaatg ctttcttcta taagttgcc //