LOCUS BG071829 709 bp mRNA linear EST 12-MAY-2010
DEFINITION H3103F06-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3103F06 3', mRNA sequence.
ACCESSION BG071829
VERSION BG071829.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 709)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG071829.1.
Other_ESTs: H3103F06-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3103 row: F column: 06
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..709
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3103F06-3"
/db_xref="taxon:10090"
/clone="H3103F06"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 222 a 149 c 142 g 196 t
ORIGIN
1 gagaacttca aacattcagt ttattaaact aaggctagag aagccatagc atgaaaaaga
61 actgcagtca ttcaggacaa agttgaggat tttataaagc caaatcaaaa gacttaagac
121 atgggaagat tcttaagtgt aaggaagtaa taaaacacag aaagtgacca caagaattac
181 aaatatattt gggtctcaga cctgggaaat ggactataca cagccttcta ggggaagaga
241 cgccttagat gttctgacag cactgcacct ttggcttgtt ctcagtggtg ggcggaacat
301 gaataggaac cacattgttg cttggagaca tgtcattttc acgtctgtct gacatttgct
361 tctgagaaac aatgcggtat atctctgtta gaattgtctg aaaagcagct tcaacatttg
421 tagaatctag agcagatgtc tcaatgaatg acaaaccatt cttctctgca aaagctcttg
481 cttcatctgt aggaactgcc ctgagatgac gtaaatcact cttattgccc acaagcatga
541 taacaatgtt actatcagca tgatctctca gttctttcag ccatcgctct acattttcat
601 atgtgagatg cttagcaatg tcataaacca ataaggcacc tactgctcca cgatagtatg
661 cagacgttat agccctgtac cgctcctgcc ctgctgtgtc ccatatctg
//