LOCUS BG071822 829 bp mRNA linear EST 12-MAY-2010
DEFINITION H3103E10-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3103E10 3', mRNA sequence.
ACCESSION BG071822
VERSION BG071822.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 829)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG071822.1.
Other_ESTs: H3103E10-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3103 row: E column: 10
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..829
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3103E10-3"
/db_xref="taxon:10090"
/clone="H3103E10"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 250 a 178 c 142 g 259 t
ORIGIN
1 acattgaaaa tagactgttt attaaaataa tttaaaagga caaacataga ctcgtgaaca
61 tacccctgta atgtaataat tccaagagaa gattgtttag aaaaatccat ttaattataa
121 catttcttta aatacaaaaa gcaatggcac gaatgaatcc ccatggcata accacagcac
181 ccattcctag tgctaccaaa gcacccatcc acaggctgct ctcgtcattg tatgctcaac
241 acttccctgt tctcatgaac agcactttct ttcctagagc ggcatcaaga gcttctgagg
301 acgtgtgttt ggcttcccct aaagaactat gaacagtgtc atcatatgcc agtggctcat
361 ccacacatgc catctttcgt cagtgacact ctacccttat gaaatagtga acattgctct
421 tatttcattt ctcttattta cagcagaggt ctgtgttctg tcacagtgtg gtctgtgcac
481 aaaaagccct ggggataata ctttgcacag catcttaaca aactgactca gattctttaa
541 aatacacaca ccgacaaaag cttgttcaca taaatgattg tcataccctg aagacttact
601 ctgaaaggag ggaaagtggc attttacaat gtattctcca attgttccag gttgtactat
661 tatgctgtgt ataatgttgc caagtaactt gatatgtgat ttttcagatt ctatctgaaa
721 gagtgttgta cacataaagt gatctagctt ttcccattgt ggtcatagat gactcagtta
781 gtccgtgtgg ctccatattg tttctcctga gtgtttaaac atttaatgc
//