LOCUS BG071763 813 bp mRNA linear EST 12-MAY-2010
DEFINITION H3102H10-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3102H10 3', mRNA sequence.
ACCESSION BG071763
VERSION BG071763.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 813)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG071763.1.
Other_ESTs: H3102H10-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3102 row: H column: 10
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..813
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3102H10-3"
/db_xref="taxon:10090"
/clone="H3102H10"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 275 a 128 c 158 g 252 t
ORIGIN
1 aaatgaatga tgatgaatga taattttaat gagaaaacaa gaagaatgtt cataggctcg
61 cacatttgtg aattgacatc ccattaggga tttgtgtttg gaggcagtag gatcgttgat
121 actgggtaca cagctgaaag agcttggtca cttcaacctc ttgggtttgg aaggttaaaa
181 actttatatt gtagacctgg gctagactgc aatcatgata catccaggtg tttcacactt
241 ctatagaaat ggattaaatt ttctattata atgcactgga atctttagaa actgtagtcc
301 agaataagcc tgttttctcc taagatattt ctgtcaagtg ttttggtcac agcaaaagaa
361 atgataatac aagttcataa gaatgtaagg ctatgaagaa gagaaggcag tggcagtcat
421 gtaagaaatg tcagatgcta gtgactcagt ggtacaaact aaagaactgc tggcattgaa
481 aaagaaagta taggtcatca aactgctggg agatctttag atagaccatt gttggtgttc
541 ctttatcata atatttcagt ggcatggcat ccagtggtct aaggatgttt ttaaatcttt
601 tttgtagtga tttattatgt caccacaaaa caacagacat aaattcgaat atcaacaaga
661 cttttgatat tattgacaaa aaagagacag tattcattga cctctatact aagttccttc
721 agccatattg tgattttcaa agcaaaacca tgaccacatt ctctgaacta ctaacctaat
781 atcaagagta catgcttcag aattcttata tca
//