LOCUS BG071750 827 bp mRNA linear EST 12-MAY-2010
DEFINITION H3102G09-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3102G09 3', mRNA sequence.
ACCESSION BG071750
VERSION BG071750.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 827)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG071750.1.
Other_ESTs: H3102G09-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3102 row: G column: 09
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..827
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3102G09-3"
/db_xref="taxon:10090"
/clone="H3102G09"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 287 a 144 c 146 g 250 t
ORIGIN
1 agtatcaaaa ataatgtgtt tattttattc atctcattag aaaagccctc aggtcaagga
61 aagctaccca ggtgcccagt gaaggacacc tgttcaccag aattgtaatt tttgctagaa
121 atctcaattt tattactggc aataattatt ggtaagtcat tttccttaaa gcaacaggct
181 aacttcattg aatcttgaga aaatgtctgc caaataacca agtctaaaaa accaagcata
241 ctttttgccc ccaagctttg tttttcaatt ccacgaagga aagaaaaccc agttcaactc
301 ataagtcaaa acaatgaagc agacgttttc ctgcagcagg ccaccgcaat cagcagggac
361 ccgagcgctg tagtgctgcc actgccacag agaataccac ctcatgcatt gtagaaacag
421 tttaaggcac agagaaatag aagcaactgg agattatgcg tgatatacgg aaagctacct
481 tctggtaata gaaagcagaa aaatgagaat gtgggagttt ctatgtttta attatagttt
541 gaatgtctta taataagatt caaattaaga aaatgaatac gagggagtct ggctgattga
601 aaaaggaaat ggctttgatt ctttcctaat ggaactctga gtgacttttt tttaacttct
661 ctgaattcag gggtattttt tcctacatta ataacagtcc atttctattt acaaaaaaaa
721 aaagttaatc caaggctttg aatatattta gctatatggt aaactgctta taaatttata
781 taacaatagt ctaccaagaa cagagttgaa ttttcagttt tttaatt
//