LOCUS BG071734 873 bp mRNA linear EST 12-MAY-2010
DEFINITION H3102E12-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3102E12 3', mRNA sequence.
ACCESSION BG071734
VERSION BG071734.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 873)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG071734.1.
Other_ESTs: H3102E12-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3102 row: E column: 12
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..873
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3102E12-3"
/db_xref="taxon:10090"
/clone="H3102E12"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 180 a 245 c 224 g 224 t
ORIGIN
1 ggctttaagt aataaaaatt ttattgagaa ttcctggcat ggtggttatc tcccccactc
61 tgggagaggc tcaaggctgt aggaaatcac ggagaaatca cattgtccca ccccgcttcc
121 cagccaacac aggaaggagg aaagcctctg aaagggctgg tctcagtctt ccttaatcca
181 agagggcaga tacagaaaag tccatcacat gatgcctggg gcagggtggg ctgtgcttga
241 cccccgagct ggctcctcca agctggacaa gtgtgccaac ttctctggtg gtggggttct
301 tgatgtccac actcatttct tacccttaat gaggctgtca cttccccgag taaaactttc
361 atcctgtaga ttcagcacaa ggttgtcagc tgtgagatag attcggttct gtgatgtggc
421 gatcgtttta gagatgttct gggcggcccg gatctttcgg agcttgatat agccaggatt
481 cttgctcagt gcttctccaa gcatcttggc agcctccgcc tccccctcag cctgcacaat
541 cttctgtcgc tgttcctgct tcgctttctc caccaaaaac tgggcccgct gggcttcctg
601 ctgggccact tgcttggctt ctacagcagc tgtgtactct cggctgaagc tcagctctgt
661 gatagctaca tcatccagga tgaggctgaa gtccttggcg cgctctgtca gctctcttcg
721 gatcaacagg gacacctgag cccgctgggt gatcagctgc gaggcattga acttggccac
781 cacactcttg agcacctcat taacaatgga cggcagcact cgctcctcat agtccagccc
841 tagacgctgg tacatgctgg ggagctcctg ggc
//