LOCUS BG071734 873 bp mRNA linear EST 12-MAY-2010 DEFINITION H3102E12-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3102E12 3', mRNA sequence. ACCESSION BG071734 VERSION BG071734.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 873) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG071734.1. Other_ESTs: H3102E12-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3102 row: E column: 12 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..873 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3102E12-3" /db_xref="taxon:10090" /clone="H3102E12" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 180 a 245 c 224 g 224 t ORIGIN 1 ggctttaagt aataaaaatt ttattgagaa ttcctggcat ggtggttatc tcccccactc 61 tgggagaggc tcaaggctgt aggaaatcac ggagaaatca cattgtccca ccccgcttcc 121 cagccaacac aggaaggagg aaagcctctg aaagggctgg tctcagtctt ccttaatcca 181 agagggcaga tacagaaaag tccatcacat gatgcctggg gcagggtggg ctgtgcttga 241 cccccgagct ggctcctcca agctggacaa gtgtgccaac ttctctggtg gtggggttct 301 tgatgtccac actcatttct tacccttaat gaggctgtca cttccccgag taaaactttc 361 atcctgtaga ttcagcacaa ggttgtcagc tgtgagatag attcggttct gtgatgtggc 421 gatcgtttta gagatgttct gggcggcccg gatctttcgg agcttgatat agccaggatt 481 cttgctcagt gcttctccaa gcatcttggc agcctccgcc tccccctcag cctgcacaat 541 cttctgtcgc tgttcctgct tcgctttctc caccaaaaac tgggcccgct gggcttcctg 601 ctgggccact tgcttggctt ctacagcagc tgtgtactct cggctgaagc tcagctctgt 661 gatagctaca tcatccagga tgaggctgaa gtccttggcg cgctctgtca gctctcttcg 721 gatcaacagg gacacctgag cccgctgggt gatcagctgc gaggcattga acttggccac 781 cacactcttg agcacctcat taacaatgga cggcagcact cgctcctcat agtccagccc 841 tagacgctgg tacatgctgg ggagctcctg ggc //