LOCUS BG071697 745 bp mRNA linear EST 12-MAY-2010 DEFINITION H3102B07-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3102B07 3', mRNA sequence. ACCESSION BG071697 VERSION BG071697.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 745) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG071697.1. Other_ESTs: H3102B07-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3102 row: B column: 07 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..745 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3102B07-3" /db_xref="taxon:10090" /clone="H3102B07" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 194 a 171 c 214 g 166 t ORIGIN 1 ctttaaatat agaaaaaaca tcctttggtt tgaatctcaa agaagcagag agtgggatgg 61 ttgtggccct ggcagggaac tctttagaga gagagacttc cacctgcaat ggagctatgc 121 ctcctcctgc atggtctcac gaatcctcga ttatccgaat agccagagtg tctgcaacca 181 ccagagaaga ctgcctctcc tgccctgtgt ctggagctaa gtatggaaag ggcagtggag 241 gtgactccca gctgtgtccg tcccattctt gctgggacaa gggtggaatg gttgacgggc 301 acatacgtgt agttcatcct gggacagttt ttttggttat actgaaaatt ggcagagaga 361 aaagagacat cccctttctc cctgccattg atcataacag ggcccaggag cctggggacg 421 agacggcgag tcctgctgga agggaggaaa aaggcagcgt gactgacagg gttgagagac 481 ccctggtcat cgagcagcaa cccgctggtt tctcataata tggggacaga aggagtccct 541 gagagcctag gaaggtcact ggagtgtaag tgtttgaaag gtggaggaga gagttctacc 601 catccccctt cccatgccct ggttctcagc taggtacctg cagctctctc tgtgtgtcag 661 agaaagggta gcaggtctag ccttagcccc tgagaatata aaatgggggc attccaagga 721 gagaaaagcc accaactgac aagag //