LOCUS BG071691 773 bp mRNA linear EST 12-MAY-2010
DEFINITION H3102B01-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3102B01 3', mRNA sequence.
ACCESSION BG071691
VERSION BG071691.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 773)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG071691.1.
Other_ESTs: H3102B01-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3102 row: B column: 01
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..773
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3102B01-3"
/db_xref="taxon:10090"
/clone="H3102B01"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 284 a 146 c 107 g 236 t
ORIGIN
1 ctcttcatga atttatttta taaaatatac ccacttaatt tgtcttctta tcactttcat
61 aatacataag aagcacagtg gacaagatgc ccattaagat gactatagtc acatttaggg
121 ggtcctataa tcaaggtagc atacatacca caacagtgtt ctaaaggcta gctcagaaaa
181 aaaaaaaaag aaaacaaaac aaaacaaaaa aacctcacgt taccttatca caatatggaa
241 cgcactgtgt tctcttttcc cattaaaata tttaagccac tgtttcattt agcattacag
301 taaataagag ctctgtctca actactacaa ggaagaaaat aatcctaaga catccttttc
361 cccttccaat gatttgaaag ctaccattat cccctgccaa ttaaaatcat caggttgatt
421 tgcactagtt gttaaaacaa tcaaggcaca agctcaacag gttatctgta gcttgtagta
481 aaatgatata ccagtatgtc tctccttagt taaaaataca taattctttt attttgttat
541 gcaacaaaag aaattaacat cacagacata gaagactagg gatgggaaac tacattaaat
601 atccagtaat ttaagcctat ttgtacttta atataggaca tgaaatcttt aacacctttg
661 aagtaaattt taatttgggg gatatacaat ttatttctca ggagtactag ttccattcat
721 ttccaggcct gctaactgtg taaataagta acacaaggca cagtagctgt act
//