LOCUS BG071620 786 bp mRNA linear EST 12-MAY-2010
DEFINITION H3101C04-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3101C04 3', mRNA sequence.
ACCESSION BG071620
VERSION BG071620.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 786)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG071620.1.
Other_ESTs: H3101C04-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3101 row: C column: 04
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..786
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3101C04-3"
/db_xref="taxon:10090"
/clone="H3101C04"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 280 a 136 c 172 g 198 t
ORIGIN
1 acttttacta aacactttgt gtttaatata ttttaaatta taaagaaatt acaaggaact
61 cctaaaaaat ttaatacaaa aatattacaa gttaggacaa ttaagcattg gtagatgaca
121 tttaaagaag catgcgggga aaggtagcat agagttaaca ttttcactgt tgacagagcc
181 ttggtacagc agagaaaagc accgcagaac caggtgtcca taatggcttt tcctctactg
241 tagttttcag gtgaatatat aggaaaaaaa aaatcaaatg aaccagtaaa ctgtaatcag
301 ctacatatta taataggtaa gaaatctaat tcatgataaa gtattagaac tatgaaataa
361 aaaatagcct ccctgcaatg gtggctcaag aaacttctgc ctagcttcag gctcatatgg
421 actcccaatt cttggcagcc caatgctttc cggaaaaaat gtaagaacag aatgtcatta
481 acaacctagg ccatgcagta aaaatgaacc ccttgaaaac taaaggttgt tagctttagc
541 agttgcagtg agggcaggac aaggctacag ctgcctgcat ctacgaagga aaaccaagaa
601 aggcagtggg cacggagaag gaacttgact aaggaaacca actacaagag ttgggggaaa
661 ctgaggctgg atgtgccttg ggatgttctt catctgttac tgggtgtgta gtaagatccg
721 aaatcagtta cgggtggaaa cactgcagca tgtttggttg gcatgggtga aagcctcagc
781 cacata
//