LOCUS BG071241 626 bp mRNA linear EST 26-JAN-2001 DEFINITION H3096D10-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3096D10 3', mRNA sequence. ACCESSION BG071241 VERSION BG071241.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 626) AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A., Tanaka,T.S., Carter,M.G. and Ko,M.S.H. TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set JOURNAL Unpublished COMMENT Other_ESTs: H3096D10-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3096 row: D column: 10 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..626 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3096D10-3" /db_xref="taxon:10090" /clone="H3096D10" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 193 a 130 c 146 g 157 t ORIGIN 1 aatgaatcat aaattttatt tcaaaatgta aacgtcacta aacatgcata catgttaaaa 61 caataaaatt tacaatttca ttaatttttt ttcttttgca taggacatta caatatagaa 121 tctatgcctt acaaaataca taacaaagtt ttatccgagc aagccaaggc cagattggga 181 aactgtacaa ctgtaatact tcactgtagt gatccaggaa agacggaaca tggcctttgg 241 aagcatgttg ggagctaggt ttcaacaaaa aagttcctat gggaaaggga actgggaagc 301 tgctggaaat ggccttggac cttggatttt gccttggggt tttggcaaag gatcccccag 361 aagttccacc gatggctttg gatgggaagc agtcacctgg gcctgtccaa caaaaccaca 421 gcagggattc ccagtcacca aggagggaca ggagctctga ccccacaatg catagtggct 481 cagcagggag ccttatggta ctgtccatgg ggtccccaca ctcggtaccc agggcttctt 541 cagtgtgcaa tgggggaaca gaaagatcct tttacccaca tactagggta gacattgtaa 601 gtcaggtgga tagtccatgg caaagc //