LOCUS BG071062 692 bp mRNA linear EST 12-MAY-2010 DEFINITION H3094C08-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3094C08 3', mRNA sequence. ACCESSION BG071062 VERSION BG071062.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 692) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG071062.1. Other_ESTs: H3094C08-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3094 row: C column: 08 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..692 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3094C08-3" /db_xref="taxon:10090" /clone="H3094C08" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 198 a 108 c 157 g 229 t ORIGIN 1 tttttttatt cttttttgtt tggatgaaat gttgtataga taccggtaaa gttcatctga 61 atcataacat ctggtagttc tatatttttt tcttttagtt tctgtcttga ttacgtctgc 121 attgttgaga agaaccacat tcaagaagca accacaccta agatgagtag actgaaggaa 181 attattgaac tcaatcaatt agaaacaaag agaacaatag aaagaatcaa caaaaccaag 241 aactggttct ttaagaaagt caacaagaca aactattaat gtgtggggtt tgatgtgtga 301 tttaagcttt aatgtttttt atgaatgtga gtatcatttt atttagagca tagatgttca 361 gaattgagat gctccattgg tccattaaag ccgtctatca tatttatatg actggattta 421 agatcacctt catatgcttc agtttcacta ggatatccag ggtttgctgt agcaggataa 481 ttattctctg gtgtggcata tcgccctggg tcatgttgac tgtgtttgga tgctgtccat 541 aaggcatctc ttttttgatg gtgtgggttg tatgatcctg atagcagcaa gacttctcag 601 gaaaacgggg agggagggtg gcatgggcca gagagtggag gtcagggtac aaactctgct 661 ggctgagcct cctgcatgcc acaagcaatt gg //