LOCUS BG070999 704 bp mRNA linear EST 12-MAY-2010 DEFINITION H3093E12-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3093E12 3', mRNA sequence. ACCESSION BG070999 VERSION BG070999.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 704) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG070999.1. Other_ESTs: H3093E12-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3093 row: E column: 12 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..704 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3093E12-3" /db_xref="taxon:10090" /clone="H3093E12" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 184 a 174 c 165 g 181 t ORIGIN 1 cttttttaaa tctttcacaa ctaacaatgg gcagtgctaa attctgcatc tgtccattct 61 cggggcaaac tcatctgtaa atcaggcctg agtttccttt ctctgtcatc tagactaaga 121 aaatccccct aagaagtcat aaacacgggg tgaaggagac agtgtgctat ggtttggaaa 181 gcgtcacctg agcagccgtg tggtagaggc ttgcctgccc tcacccccat tcagaggagg 241 gagaactttt gaaaaagtga agccacatgt gagtgaagcc actccgtctg ctacctgacc 301 acggtaagtt gctaagttgt tattttcggt ttctaggaac cggtgcacaa gtctgagaga 361 ctcctttgct cccaaggaca ctctctagta aatggctctc catccagctg aggagagttc 421 tgggtctgcg aattgactta gggttggaaa aatggctcca actataactc tcagttctga 481 caactaaaag tcacccatct gttacattcc tgaccactag aaatctgccg aaaccatctc 541 ggtcaccagg aatgacacgg aagacagtgc tttctgatgc agctccaccc tccctgcctg 601 ccatgaggaa ggatatatca gactgcttct tcttcatggg tgtgtctcac aacacctggg 661 gaaggaatgc tgacagactc tatggaatgc agctgcaggg tgag //