LOCUS BG070976 888 bp mRNA linear EST 26-JAN-2001
DEFINITION H3093C12-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3093C12 3', mRNA sequence.
ACCESSION BG070976
VERSION BG070976.1
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 888)
AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A.,
Tanaka,T.S., Carter,M.G. and Ko,M.S.H.
TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set
JOURNAL Unpublished
COMMENT Other_ESTs: H3093C12-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3093 row: C column: 12
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..888
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3093C12-3"
/db_xref="taxon:10090"
/clone="H3093C12"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 231 a 218 c 218 g 221 t
ORIGIN
1 catgacgagc atgtctgtcc agctatcaat catgtttgtt tgcaccaaat cccaagccat
61 taaaatacga ctaattttaa gttaaacaga agtcgtctgc tccaaatttg ccatcaacta
121 tccatgctac tgcattcctc ttcaatattc tggtggtcta tgaaaggtgc tggtccccat
181 attctaacag tgccatcgtc tgaggcactg gccatcatgg atggaatctg tgggttccag
241 ctaacacaat ttactgtgcg cgtgtgccct gtgagctccg caattggcag ttcactacgt
301 ttgtgccaga tgtaaacctt gtgatctaga ctggccccct gaatctccag acaaccaata
361 tcacttaaat aagtgatagt cttaatacta gtttttagac tagtcattgg agaacagatg
421 attgatgtct tagggccgga gaaacgcaga cggcgtacca cacaggaaaa gatcgcaatt
481 gttcagcaga gctttgaacc ggggatgacg gtctccctcg ttgcccggca acatggtgta
541 gcagccagcc agttatttct ctggcgtaac aataccagga aggaagtctt actgctgtcg
601 ccgccggaga acaggttgtt cctgcctctg aacttgctgc cgccatgaag cagattaaag
661 aactccagcg cctgctcggc aagaaaacga tggaaaatga actcctcaaa gaagccgttg
721 aatatggacg ggcaaaaaag tggatagcgc acggccctta ttgcccgggg atggggagta
781 agcttagtca gccgttgtct ccgggtgtcg cgtgcgcagt tgcacgtcat tctcagacga
841 accgatgact ggatggatgg ccgccgcagt cgtcacactg atgatacg
//