LOCUS BG070935 670 bp mRNA linear EST 12-MAY-2010 DEFINITION H3091H09-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3091H09 3', mRNA sequence. ACCESSION BG070935 VERSION BG070935.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 670) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG070935.1. Other_ESTs: H3091H09-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3091 row: H column: 09 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..670 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3091H09-3" /db_xref="taxon:10090" /clone="H3091H09" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 163 a 177 c 138 g 192 t ORIGIN 1 ggtttttcgg gcttaaatat cttaactact tgagtgaaat aaagactttt cccccacata 61 tctgctgtcc agccagctct cacctgttag ggcattttct gatgtagaga gctgctctcg 121 aagtttatcc acatcatctg ggtgcacctg atcatacagt gtgctcccga accattcaga 181 ctgtggctgg ttcaaaacgg gagtcactga gtcagagaca tacaccaccc gtccagtctc 241 acaggagaca ataaacagaa agccatctgc tgcctccaag atcaaatgtt tcagttcctg 301 atcagtgagg aaagatggct tgtaggagcc atcagtagat gtgttgccag ttcccctcaa 361 ggacttcatg tgagaaacgg ccatgcgtaa gatggttagc ttgtctggtt ttcgagccag 421 ggcactacat gtaggtacca tgtctgacag ttctgtgatg taagctgtca tcttgttccg 481 tcgccgccgt tctatttcac tatgattttc cctggcaaac cgctctttgt cattacacat 541 ctggtcatca tcgcatctca aaaatttagt gttcacttct acttcatcat caaaatccag 601 ccctgaccgt cgcttaatag ccctctgtac aacagctcct ccaccttgaa tcccaggtcc 661 agggtttcca //