LOCUS BG070930 542 bp mRNA linear EST 12-MAY-2010 DEFINITION H3091H02-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3091H02 3', mRNA sequence. ACCESSION BG070930 VERSION BG070930.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 542) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG070930.1. Other_ESTs: H3091H02-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3091 row: H column: 02 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..542 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3091H02-3" /db_xref="taxon:10090" /clone="H3091H02" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 144 a 149 c 116 g 133 t ORIGIN 1 attttatttc agaagtacca ggttattagg aagcatggga atatctaaca tcattctttc 61 atatctaggt ataggatcac cctatctgac tacttcaata tcctctcctc tctctctaga 121 catctactag gacagtctaa ctgtatagat cctaatttgc cttccttaga taatctatga 181 acccaagcac tcccatccca tcccacaaaa gcacaaaagc ccactcccag gcagcaaaag 241 ccacagggat atccggaaaa gcagccaggc actgcagggc cctcatcaca ggcaccagca 301 gcatgggggt ctctgtagag gatgggcgct atgtctgctc tgcattctcc caagtccatc 361 agagcctgcc ggaccaggtc agctgctcat gtggtctctg cccaggataa ctgctttcaa 421 ggctcccaag gaagtaagcc tttgctgccc aactgcacgt tacaagatgg ttctgctgtc 481 cctatggaac tcaggtaagg agtagatatg gaggatcaga tttaccctgc ctggtggcag 541 ag //