LOCUS BG070910 717 bp mRNA linear EST 26-JAN-2001 DEFINITION H3091F06-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3091F06 3', mRNA sequence. ACCESSION BG070910 VERSION BG070910.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 717) AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A., Tanaka,T.S., Carter,M.G. and Ko,M.S.H. TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set JOURNAL Unpublished COMMENT Other_ESTs: H3091F06-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3091 row: F column: 06 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..717 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3091F06-3" /db_xref="taxon:10090" /clone="H3091F06" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 172 a 195 c 160 g 190 t ORIGIN 1 aaacagtaat tccagcaggg gccctcctgc tgtgcctgat cctggctaaa gagtgtggac 61 actctcggct ttgcttatac cagccatctt ttgttttcta acatgagcac agaccattca 121 gacattcaga cgactagaca cgaacacaca tgaattgaac acgtggacag actggtgcac 181 cagacattag agaagacctt agacaaaatc acaaagaggg cagagaactt ctgctgtagg 241 gcccagagag aataaagcag ggagtccctg attctctctg tctctgggag agttttcctc 301 ccacctgatg cagttcttag actgcggcca atccgcctga ttattaagtc caatcccttt 361 atctctctca cctaaaaagc agcttctgga agctgcaccg actgcctgtt acgttcctag 421 atcctgatat cactgctgca cctaagcgag tccagcacgt gggttcagca ctgttccagc 481 ttagcccgta ccacacctac caaccttttt gcctgttgct tctcgtgttc tggtacgaat 541 tttctttaat tcttttcttc ttccacggag ctccaaacta gcagtgtttc ttccaaatgt 601 cctctgccat tcccaggccc cgcgtgggat gccaatttgt tggggacggc ctgcggctct 661 catgtccggg ttcgaacctg ggagatatct tggagctgaa agaaaaggga tagtcga //