LOCUS BG070860 828 bp mRNA linear EST 12-MAY-2010
DEFINITION H3093A06-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3093A06 3', mRNA sequence.
ACCESSION BG070860
VERSION BG070860.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 828)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG070860.1.
Other_ESTs: H3093A06-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3093 row: A column: 06
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..828
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3093A06-3"
/db_xref="taxon:10090"
/clone="H3093A06"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 250 a 169 c 178 g 231 t
ORIGIN
1 ccatagaaca ccaccaattc tttattatta ttattattaa aacagattaa gttcagtctt
61 gtcagcatag aaaccagcaa ggcaaggcta gttgacttgt gtctaaattg gtgacagttt
121 taaagtgttc atacactgac ttgtgtctta accagcaagt tacaggtctg agcttctgtt
181 ctcagtgact cctgaaacac agaaactgtc tggtagaaac tgatagttga aaagccatct
241 acagtaagtg ccataggctc atccccctgc ctcagccctc agagtgctgg gatttctgag
301 ctgtgatgac acaaaacctt agctcttatc tcaaaggaaa tgaaagatca tttattacgg
361 atctaaacat taggtgatca cggaatagtc acatggattt ggttgccccc aataacatgt
421 tcaaacatgg tgggagcagt ttcatagagt ttttatagta tcagaacaaa ggcactcaaa
481 aatcaaagta tttgaaaaat catctgtgga aatgccaggt aggcaggtta tatatagtaa
541 ggcagaaaaa gatttttatg ggcctaagag gctacttgat gacattttta gcctttggag
601 gaagtggaaa cttggggttg gtttgtaccc caaagggatt cacttaatag gtgatacacg
661 gctattaagg ggttaaaggc acagctctgg cctcagacct gcaactttcc agctctcaac
721 agcacagaag gtcttgccag tcagtcacca ctgaggaatc tttaaaacag gtgaggcttg
781 gctcccctcc cttgcccacc tctgagtttc agttcacaga tgtcatag
//