LOCUS BG070811 601 bp mRNA linear EST 12-MAY-2010 DEFINITION H3092D07-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3092D07 3', mRNA sequence. ACCESSION BG070811 VERSION BG070811.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 601) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG070811.1. Other_ESTs: H3092D07-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3092 row: D column: 07 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..601 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3092D07-3" /db_xref="taxon:10090" /clone="H3092D07" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 184 a 123 c 141 g 153 t ORIGIN 1 gttaaattta aaaatttaat catagtcctt gggcagtaga ggcaggcaga tccctgagtt 61 tggtctacag agttctagga cagtcagagc tacatagaga aaccctgtct caaaacaaaa 121 aggggagggg agtgctggtg aggtggctca gggattaaga gcactgactg ctcttctgaa 181 ggtcccaagt tcaaatccca gcaaccacat ggtggcttgc aaccatctgt aatgaaatca 241 gacgccctct tctggagtgt ctgaagacaa ctacagtgta cttacagata aataaatctt 301 tagaaagaga caatccacga gctgagatcc ttcagaggct accttctgta caacagcacg 361 ccaattaatg ttttccaggg ccgaacagcc aggactgaga aagtacacag tgatggcttt 421 ctgctcagac atgtcatacc agtgttttga agaataagag aaaagggcac cagctaaata 481 ctggttacca accctcagag actgaatgaa gggcaatgtt ctatttttat ttgtatgtgt 541 gagactgggt ctctccctat atagctctgg ctgtcctgga atttagactg taaaatttga 601 a //