LOCUS BG070796 781 bp mRNA linear EST 12-MAY-2010
DEFINITION H3091C05-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3091C05 3', mRNA sequence.
ACCESSION BG070796
VERSION BG070796.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 781)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG070796.1.
Other_ESTs: H3091C05-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3091 row: C column: 05
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..781
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3091C05-3"
/db_xref="taxon:10090"
/clone="H3091C05"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 266 a 144 c 159 g 212 t
ORIGIN
1 ggataagata aaatctttat ttacaatgat ctggattaaa atatgttaaa aagcaagaaa
61 acaaaaggat gagttctggt gtaaaaaaaa aaaaaatcaa gagaaccaga tgatgctatg
121 atgttcaaaa ctgaaaagca actggagtta gccacagaga cctaagacgt gaggaacaca
181 cggggaacag gtacaccaaa attcctgggt cctctgcctc tgctgattgt gtttcacaaa
241 ttaataagca gctcaggaat gtccaaatta tagtcttact ctagttgttt cacacaatga
301 tagtctttta tttaataatg caaaatcaga aaagagattg cttccattca gaaagtatag
361 taggcaaatt ccttgctcaa aggagctggt gattggagga gcagacagat cttggaaagc
421 actctgaaat acaggcaaca ttctcttgga agctggcact taaagccata ttttatccct
481 ttttatgtac agggaaagtt ctttggaccc cagtgaagaa taagtaacgg cagcttctta
541 atccataggc caaagtagca catttctgct tctcctttca gttcagagcc atgttttctg
601 attattcatt aggagtcctt agtaagcagc tctagaaata catcactagg gaaaagaaga
661 ctccagtact tacgatacac aagaccgttg ctaaggagaa accctggaag ctcggtgatc
721 ttacctatag atgccacatc aggatggctt ttgagatctt tgtacattca agctggttca
781 g
//