LOCUS BG070780 850 bp mRNA linear EST 12-MAY-2010 DEFINITION H3091A12-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3091A12 3', mRNA sequence. ACCESSION BG070780 VERSION BG070780.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 850) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG070780.1. Other_ESTs: H3091A12-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3091 row: A column: 12 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..850 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3091A12-3" /db_xref="taxon:10090" /clone="H3091A12" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 235 a 193 c 197 g 225 t ORIGIN 1 aaattctaga aaagctttat tagttcctat ttgtgatgat aatcttcaag ggttattgtt 61 gtaccaggaa agacagtttc cttgtatgct gacctgattc atgcacaccg gccttttgtt 121 ttgtgtggtg ggaggtcctt ccctcgttgg tttgcatttc attttcctga ggtagagaga 181 aggctgaggg ctcatcttgc ttgttcttca cggtcctggg atgcttagat tcagatgagt 241 ggacttcctt aaagactatt tttcagcagg gcaattgcct ttaatcccag gactctggag 301 gcagaggcag gtaaatctct tgagttcaag gccagtctgc tccacagagg aagttcaggg 361 atatactgag gggccttgta ttgaaaaaca aaacaaacaa caacaaaatg actattttcc 421 agcccatagt ttggctacag tgggataaga gtagtggcca gccaaaagct gcaaggcaca 481 gatgagcaag tctgccttgt cccaataact gtttggtgcc acatcctgag ctgtgagcct 541 gccctgcctg tcatgggcgg ggaggagagc agctctgctc acgaatgaaa agctcttctg 601 tttacctgag aggtccaggc taatgtcaca ggcacatgac tagtgcttca tcaccatgac 661 acctgtcata agccttccaa ggcaaccaaa gaactaccca gtcttcaccc ccatttcaaa 721 atacttacag atgagtctca taatgaaaat tctctagaag ccactaactt ttaaactaag 781 gacaaagtag acggaacaag ctggagagta agaccagtgt ccctaagcac caacccaaag 841 ggccctgatt //