LOCUS BG070778 674 bp mRNA linear EST 12-MAY-2010 DEFINITION H3091A10-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3091A10 3', mRNA sequence. ACCESSION BG070778 VERSION BG070778.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 674) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG070778.1. Other_ESTs: H3091A10-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3091 row: A column: 10 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..674 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3091A10-3" /db_xref="taxon:10090" /clone="H3091A10" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 200 a 185 c 110 g 179 t ORIGIN 1 gaatatataa ttaacttgtt tccactccca cctcccagat tgtaattctt gggagaaatt 61 gtagccttcc cagaccttgg aatctgcctt gcccagaaat tctaccacta ggtcggatta 121 gtgtagacaa aagagaccaa catcaaaggc attgcctgtc acagatacct ttgccagaat 181 cttacaccag caagagtgta atcttatgcc ttacaattct agagattgcc aatatggtcc 241 acaattcaaa gttccttgac tatcttttat taatttttaa aggccgatga aacacctgtc 301 agccacccac ccaccaccac caccaacact gcttttccaa actgctgctt tcccaaagca 361 tttaccaatg gaggtctgcg aagttatgaa taccaagaat tctcacaaaa ccacaaaaca 421 accaagcaac tgtcaaagga aacctcaaga agtgaggaac ttccacaaaa gtcgtatcat 481 tcattgtggt aatccaaaga ctgctccctg attccggatg aagctccagg tctcgatccc 541 tcagaggatg atcccggatc tgcagtagca tcatcttgcc tttaatttga tccaaattgt 601 tcttactccc cacttttctg atcatgtcca tcacccacag ccttgcctta agtggcccca 661 tcacagtgac tcgg //