LOCUS BG070727 583 bp mRNA linear EST 26-JAN-2001 DEFINITION H3090E04-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3090E04 3', mRNA sequence. ACCESSION BG070727 VERSION BG070727.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 583) AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A., Tanaka,T.S., Carter,M.G. and Ko,M.S.H. TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set JOURNAL Unpublished COMMENT Other_ESTs: H3090E04-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3090 row: E column: 04 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..583 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3090E04-3" /db_xref="taxon:10090" /clone="H3090E04" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 143 a 128 c 128 g 184 t ORIGIN 1 cattttaaag gatctctcct tcagcatgag gtttttggtg ctttgctttt ggacctctct 61 ttattgagtc attttctagt ttacaaagac ttaggagtac aaaaacgtgg gagtacagag 121 agatgttaca tcttttctgt ttttctcctt tgagtctact gagcagacca tttttcctgt 181 gtggtgcatg atgaggagtc gcagtcaccg agggtactcc gaacccttgg ctgatgtcag 241 cggacagctg caggtgggaa cgggtcctca gtgatggtta cagatgccag atgattctag 301 aacgctgaat cctgaattaa gaatctagat cagtgttctc aaccttagtg atgtgttgtg 361 ttggccccca accataacat gattttcatt cctacttcat aactgcaact ttactactgt 421 tattaatgat catgtaagta tctggtgggc tggatatttg atatctgacc cctgtggata 481 ggtcattcaa cctccccaag gggtcaagac ccacaagtta acacaggtct aggaagtatg 541 actacacctt ctccgctgct ctgattccac gcattccaca aat //