LOCUS BG070612 596 bp mRNA linear EST 12-MAY-2010 DEFINITION H3089C01-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3089C01 3', mRNA sequence. ACCESSION BG070612 VERSION BG070612.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 596) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG070612.1. Other_ESTs: H3089C01-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3089 row: C column: 01 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..596 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3089C01-3" /db_xref="taxon:10090" /clone="H3089C01" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 131 a 150 c 147 g 168 t ORIGIN 1 ggtttttcga gacagggttt ctctgtgtag ccctggctgt cctggaactc actctgtaga 61 ccaggctggc cttgaactca gaaatccgcc tgcctctgcc tcccaagtgc taggattaaa 121 ggcgtgcgcc accagcccgg cttgattttt agctttctta tatagcctag ggtcatctgc 181 ctagggttgg tgccacccac ggtgggctat gccctcccac accaaccatc aatcaagata 241 attcctcaca cacatggcca tggtccaatc taatctgggc aattccccaa ctgaggttcc 301 ctcgactctg ggctttgtaa agttgacagc tgcagctaac taggacaagg gtcacatatg 361 gtttctgttg aaacactgta ggtctgttgt tgtagttaag atttatttac tgtattcatt 421 agtgttttgc ccgcatgtat gtatttgtac catgtgcaca gctggtgtac ttggagagag 481 tgtcagattc cctagaacgg gagctgtgga tggctataag ccactgtgtt gagcctggga 541 agtgagtcta ggtgaccctc ggcaagaaca gcaagtgctc tttaacaagc cccctc //