LOCUS BG070603 640 bp mRNA linear EST 26-JAN-2001 DEFINITION H3089B04-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3089B04 3', mRNA sequence. ACCESSION BG070603 VERSION BG070603.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 640) AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A., Tanaka,T.S., Carter,M.G. and Ko,M.S.H. TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set JOURNAL Unpublished COMMENT Other_ESTs: H3089B04-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3089 row: B column: 04 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..640 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3089B04-3" /db_xref="taxon:10090" /clone="H3089B04" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 178 a 122 c 152 g 188 t ORIGIN 1 ggctgatatc catttattag tgagtacaca ctatgcatgt cctttcggat ctgagttacc 61 tcacctagga tggaatttgc tagttcagtc catttgcctg caaagctcat gatgtcattg 121 ttctcaagag atgaataata ttccattgtg aaaatgaacc atatttctgt atcagttcgc 181 ccattgtggg gtatctggtt tgtttccagc ctctggttat cacaaacaag acaactctga 241 acatagtgga acacaagccc ctgtgtcatg gtggggcatc ttttgtgtat atgcccaaga 301 gtggtatagc tgggatttca ggtatatcta tttccaattt tctgaggaag ctccagattg 361 atttccagaa tggttttacc agtttttgtg ggaagccaca tgtgccgttg ctgagtggca 421 ctgactactg ctggccacca cgcataagat tggacaaaca accaatgtgt acatatgcag 481 taaagttttt tgcaaagaca cggcctggcc caggcatgat aatgaggttc agtaaggtac 541 tgagagtata accaatcaga tgtgagacat gcaaatgagg tatgataatg aggttctgta 601 aggtactgag agagagtagc caatcagatg aggaacatgc //