LOCUS BG070580 716 bp mRNA linear EST 12-MAY-2010 DEFINITION H3088H05-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3088H05 3', mRNA sequence. ACCESSION BG070580 VERSION BG070580.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 716) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG070580.1. Other_ESTs: H3088H05-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3088 row: H column: 05 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..716 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3088H05-3" /db_xref="taxon:10090" /clone="H3088H05" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 234 a 142 c 139 g 201 t ORIGIN 1 agtcacttat atataggtga ctatctgcag gaatgtgcac atgaatgcag atgccagtaa 61 actacaaaag agagcgtcgg attcccctgg acttgagaca caggtttcac ctaacatgag 121 tgctaggaac cacactcagg ttctctgaaa gatcagtatg tactcataac cactgaacca 181 tgtatttctt ataaaggtgt gtatgctaaa gtggagatac tcattaattt atgtcacccc 241 actgcaacct aaagctgata agtattatat caacacaata atcctttatg tattagtcct 301 gaccagtatt tgaactggtt aagcccaata gttggtataa aggtatatag tacaaactat 361 attcaccatc tctccttgag catcatttct tgctctgaaa aaggaaagcc gtagacaacc 421 tgaaggtact ttcaggcagt ctgtgacctg ctgccacagt gagtttggct ttattatggt 481 ctgaaagatc aatttcctat ctcaatggaa ttttttgtaa gggaatagga aagaggaaca 541 aagtaatcac acactagttg aagctgtatc acaaaacagc caagtttcaa aatggttcca 601 tcactatcaa aaggaggcaa cttcccagag aatgcatgga cagtagtgtg ctggcagctt 661 cacttagtat tattactgaa gatgccacaa acttaaggtt tcaatatcta cagacg //