LOCUS BG070520 704 bp mRNA linear EST 12-MAY-2010 DEFINITION H3088C03-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3088C03 3', mRNA sequence. ACCESSION BG070520 VERSION BG070520.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 704) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG070520.1. Other_ESTs: H3088C03-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3088 row: C column: 03 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..704 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3088C03-3" /db_xref="taxon:10090" /clone="H3088C03" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 104 a 188 c 130 g 282 t ORIGIN 1 ccctcttgat cgtcttcgtc ttctgaatca gaggttgatg taacccctgg tttagctaaa 61 tttttccgtt tagattcaac ttctttcttc ccttctttct tatctggttc ttttcttggc 121 ttctcttctt ccttttggcc ttcttcctct tttttaagct gctttttagg ttgtttctcc 181 tctggtccct tttttttact tttatcttca tcaataacca tgtcaccgtc tgaaggacaa 241 ggctgcttta ccactttagg tctgcctctt ggtttgggaa tcttgacttc agtagctgca 301 ggtcgtcctc tcttaggact tgctttcaca ttagaagcgg ttgctgcagt caccattccc 361 gcctcttcag tgtctacttg tttttcagcc tttctctttc ttcctcgcct ggcagctttt 421 ggagtggtta agcagccttg tccttcatca ataaccatgt caccgtctga aggacaaggc 481 tgctttacca ctttaggtct gcctcttggt ttgggaatct tgacttcagt agctgcaggt 541 cgtcctctct taggacttgc tttcacatta gaagcggttg ctgcagtcac cattcccgcc 601 tcttcagtgt ctacttgttt ttcagccttt ctctttcttc ctcgcctggc agcttttgga 661 gtggttatgt caactgcttt agtcacatcc tcattgctgg cctt //