LOCUS BG070490 757 bp mRNA linear EST 12-MAY-2010
DEFINITION H3087H08-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3087H08 3', mRNA sequence.
ACCESSION BG070490
VERSION BG070490.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 757)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG070490.1.
Other_ESTs: H3087H08-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3087 row: H column: 08
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..757
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3087H08-3"
/db_xref="taxon:10090"
/clone="H3087H08"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 230 a 177 c 183 g 167 t
ORIGIN
1 agctgatgaa caaggaagat gagagcaaga aggtcccagt gtataggatg gcagtttgtg
61 taaggagaag tgctgagaaa cttaaaagaa ctagtttgaa aactgttaga agggtgtaag
121 acatgtgtac acacctttaa tctcagcgcc agggaggcag aggcaggaaa atctcttgtg
181 agttcaaggc catcctggtc cacacagcaa gttccaggac agccagagct acatagtaag
241 ctgtctcaaa acaactgcta gagagacaag ctcatgccgt cctaccactg cccaagatgt
301 cagtaacgta taggaagggt ctcagccagt gcatgacaca gactgactct aactcagaca
361 tggctgggtc ctggatccat cacaaatgat tggagcaagg accaagattc tcctgaagac
421 ctgaactagg ttctagtaag agaatgtgag ctgtgatctg cctctgagca gccagctctg
481 cccctgaaga ggctacgtca cctctgctcc tcagcttctg caactctgag gccagagcat
541 gtggaaacca gtgtgatctc cttacaaagt aaagcacatt tgcacgtctg cagcttcagt
601 aagatacact tatatctgga catttctggg aagaaacccg aagaacacaa agctccgcca
661 gggcactgtt tatttaagga agcagctatc agaggcagtc cttacctttc tcacgtatga
721 caccaattct cctgaataat actgagacac actgagg
//