LOCUS BG070476 669 bp mRNA linear EST 12-MAY-2010 DEFINITION H3087G05-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3087G05 3', mRNA sequence. ACCESSION BG070476 VERSION BG070476.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 669) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG070476.1. Other_ESTs: H3087G05-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3087 row: G column: 05 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..669 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3087G05-3" /db_xref="taxon:10090" /clone="H3087G05" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 193 a 134 c 135 g 207 t ORIGIN 1 aaaaactttc ttaatagctg agccatggct caagcctctc tcctttatgg ttttaggaca 61 agatcttaat tatgtagcac aggctagtca gcagtctttt ttttttaaat aaagatttat 121 ttatttcatg tatgtgggcg cactgttgct gtcttcagat acaccagaag agtgcattgg 181 atccctatta cagatggctg tgagccacca tgtggttgct gggaattggg actcctttgg 241 aacagtagtc agtgctctta accactgagc catctctcca atcccagcat tcgggtctta 301 aatcatccca gacttctcag tgctgggatg acatgtgtga tgagccacca catgggccat 361 acatgtactt actgttcact gaggtatcat atcttttgaa aaatgaatat acaaccagca 421 tacaccatga tttcaataca tttctgtatg tatttctggt atttgaggat ttaaagatat 481 atcaatttta tcaaagacaa cctattaaaa catgattaac atatactaga aataagaatc 541 ccctgatgaa taagcctggg gaatgactcg gtgggtaaaa tatttgctgt ccctcaacct 601 gaggacctga atttgggtag ccagcacact taggtggtgg gtaagacaaa tctgtatccc 661 aaatgctgg //