LOCUS BG070450 587 bp mRNA linear EST 12-MAY-2010 DEFINITION H3087E03-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3087E03 3', mRNA sequence. ACCESSION BG070450 VERSION BG070450.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 587) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG070450.1. Other_ESTs: H3087E03-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3087 row: E column: 03 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..587 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3087E03-3" /db_xref="taxon:10090" /clone="H3087E03" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 131 a 132 c 158 g 166 t ORIGIN 1 cagttctcac acctttattc ctccaggata cgattcttat ctacagtgct ggggcgggtc 61 agggtctgac agcaggtact tctcattggc ttggagatac cttatctgca tgtgggaggg 121 cttggcactg ctcctatgtg actgatggcc actgagctct ctggggcaga agtcatcgtg 181 ggaggctgta actgcgtgac aggagctcaa ggggcatggc caaatgcctg ctgttccttg 241 caggtgacag tcacctgctg ggtcactggg gttttaagcc tgagctgggc caggcaccgg 301 actgcctatc acagctcacc accccatatt acttcatata gaaggtctcc aggatgctct 361 gcagccatac acctatctga gcacagtcag acttggactg gtcatagtcc tgtagtagaa 421 ataacttgca agtactcgtg ttgttaaatt tatgagcagc tgagtataga cagatcttgt 481 agttaaggac caagttatag acgtgttgta ggaagcagaa tcatgtgagt ctcttcatga 541 ggtgagtgtt gtcatgttcg ccattcttct ttcatgggat gttttag //