LOCUS BG070428 691 bp mRNA linear EST 12-MAY-2010 DEFINITION H3087C04-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3087C04 3', mRNA sequence. ACCESSION BG070428 VERSION BG070428.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 691) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG070428.1. Other_ESTs: H3087C04-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3087 row: C column: 04 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..691 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3087C04-3" /db_xref="taxon:10090" /clone="H3087C04" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 185 a 160 c 166 g 180 t ORIGIN 1 cagttttggt ttgtttgttt gtttcagctc tgagaggtaa gggagaccac aagggcctga 61 ctcaaagctg ccagaagcca tatgtgggaa ccaattcagt atctgccccc taccaccacc 121 aaaccttctg caggccctgg cttcaggacc cgaattcaaa tagtgacctc agacatctct 181 ttgcaatcta ggaatcctgg caaggcaggg tgtcatatga ggtgttgaaa atgatgggcc 241 aggttgtctc tgtgcccaga actccaaggt cattcagcag tgtcaccaac cagtgggtga 301 ctacagtgga tggttcatca ggaccaggac aggcagtgga gagcatcttc cctctagaca 361 gagagagtcc tagctggcag gtacacacag atgcttgctt gcctgtcaca ttgcctcctc 421 ctctatcctt cacttcagat atggattgag atttaaaaag cacttctagg cagtatcaac 481 tgtcaagtgg ccaagcatgg ttctcaagat ttctagctat aagtgtagct caagactaga 541 atatgttcct tgttagaggg aggccctatt tccaatcccc agccccacag agaggtggag 601 ggatagtact tttaaagttt tccaagtgca atcaatcagg agtcttttta attataggga 661 agttgaaata aacccgcata aggtcatgga t //