LOCUS BG070416 756 bp mRNA linear EST 26-JAN-2001 DEFINITION H3087B03-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3087B03 3', mRNA sequence. ACCESSION BG070416 VERSION BG070416.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 756) AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A., Tanaka,T.S., Carter,M.G. and Ko,M.S.H. TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set JOURNAL Unpublished COMMENT Other_ESTs: H3087B03-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3087 row: B column: 03 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..756 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3087B03-3" /db_xref="taxon:10090" /clone="H3087B03" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 222 a 200 c 137 g 197 t ORIGIN 1 tttttgaaac ctaatatggg gccggtctac aagtacaatg gcacatccca ctatcaccaa 61 gatgggacca tggcatcatc cagacatgca tggatcaaag ctgcggagtt ccaaatcttc 121 aactcaaagc agctaatgaa attccaagca gctaggatag aggatcttaa agcccatgcc 181 acaatagact cacctactcc aataaggaca cacctccaaa tagtgccact ccctgggcta 241 agcatatgta aactaacata ttccactcct aggcccctat aggcatgttc aaacaaagga 301 ccctaggggg gctatatcta ccagagcata ataaaaaagt acatttagtc caagttccaa 361 agtcctcata gtctatagca gcctcatcaa tgttaaaatt ccaaagttca aagtatcttc 421 tgaggtccat ccaatcactt aactgtaatc ccaaaccaag acaggaaacc agctgggcag 481 actccaaaca ctgcatcttc atgtctgatg tcaaagcagt tttcagatct ccaactcctt 541 ttgtagcttt gttgactgca ataaagttct ttcactaggc tggttctact ctgtcagtag 601 ctttcctcag gagatatccc atggctctgg catcttgaaa attgtggggt cccaaggcag 661 cttcaatgtt acagcttttt gtgtcaatgt ctgggatcca cacttgctct acttggctcc 721 tccaaagggc tggcatcact tctccaactc tgcccc //