LOCUS BG070399 746 bp mRNA linear EST 12-MAY-2010 DEFINITION H3086H10-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3086H10 3', mRNA sequence. ACCESSION BG070399 VERSION BG070399.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 746) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG070399.1. Other_ESTs: H3086H10-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3086 row: H column: 10 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..746 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3086H10-3" /db_xref="taxon:10090" /clone="H3086H10" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 181 a 173 c 140 g 252 t ORIGIN 1 cgagacaggg tttctctgta tagccctgga tgtcctggaa ctcactttgt agaccaggat 61 ggcctcgaac tcagaaatcc gcctgcctct gcctcccgag tgctgggatt aaaggggtac 121 gccaccacac ccggctacac cttttacttc taattatttg tatctgtaat taagcacttc 181 tcatgggctt catcaagcac aatgacatgc tcacaaactc cagacatctt taagtgtgcc 241 tccatttgcc aacagatgtt tatgttcttt gtcttctgga gaactgggtc tttattaatc 301 tgaggtcttg ccctagcttc tatcctattc tgctgaccca tataacctct acattctaaa 361 tacaactgtg tatatccaca gtgatctata gtcttataac actcataaaa ctggcttgtg 421 tgttgaaatc acagatggcc ttatgagtta tagagaggac cattttcaat cagggccttt 481 ggaagatcct tgcatcacac agcctgaaat ctggtttttg tggggtttgt tcgtttgttt 541 tgtttgacaa ggtctcatgt agtccagact gaccttcaaa tctttgggat aactttgaat 601 ttctgatctt cctgcttctc ccatttaatt gtgggcatgt accatcacag tttatgaaat 661 attaggattt aaatccttgc atgtgctagg ttagcactga actataccta caacctagat 721 ccttggttgt cttcattcct tcatta //