LOCUS BG070365 721 bp mRNA linear EST 12-MAY-2010 DEFINITION H3086E11-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3086E11 3', mRNA sequence. ACCESSION BG070365 VERSION BG070365.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 721) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG070365.1. Other_ESTs: H3086E11-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3086 row: E column: 11 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..721 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3086E11-3" /db_xref="taxon:10090" /clone="H3086E11" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 201 a 166 c 176 g 178 t ORIGIN 1 ctcaaacggc tacacccaac acagtgattt ttttgatccc taaatcatga gaggggtagt 61 ggatgctgaa gaagacaaat ggctttggaa ggtcagatag aaaacacact caaaggagga 121 ggccatgagt gctgaagggc tgcgagcacg gtgtgctttg agctaccagc caagcttgga 181 ggcctcctct gacagagggg tgggcagtcg cggctagcgt acctacctgc cctcaacctt 241 ttctaaaaaa tggcatcttg accgtaacat gtaccttcct ggaaacagga gataccaggg 301 cagatttatc gcttaagtta gctcttaaaa gggcatgaga tttcagctca cttccaagtg 361 atttcatccc ttatccaagg ctgcccattc ctttgggacc tgcagtgata ttgttccata 421 tcccttccat aatgtgcact tgggtgctct actgtgtatg gaaagctctg tcacaggatc 481 ctgagagctg cagggagcat tgaaatgcag agcaaatgga aaaaaaaaca gccttttggg 541 ggttattcat atgggatcag agagagttgg taaatacaag gaaatctaaa aataaccaga 601 gtccttaaaa agaagaatac caaagagagc aagtgtgccc ctgctccact gccattcctg 661 tgaaaccagc cctggaatgc ttgcgcctga gctcctggct ctttcattcc tgctcttttg 721 c //