LOCUS BG070359 737 bp mRNA linear EST 12-MAY-2010 DEFINITION H3086E05-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3086E05 3', mRNA sequence. ACCESSION BG070359 VERSION BG070359.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 737) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG070359.1. Other_ESTs: H3086E05-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3086 row: E column: 05 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..737 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3086E05-3" /db_xref="taxon:10090" /clone="H3086E05" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 188 a 181 c 178 g 190 t ORIGIN 1 caaggccaga ttttccttta ttaacttcag tgtgcacaga acacagttta gccatgtaaa 61 gccttgggtc ctccccaagt acagtcaggc agcttgaatg gctttgggac gcttcctaag 121 tcaccagaac aagcttgttg tcgctcagct tgtagcgaaa tgtagcaccc cgattgaaaa 181 gatctcctgt cactctgtct tgcttcctta gggctggcta ccaaaaagct agctctccag 241 cccttggccc aggtccttcc cggtggcaac tgaggaactt tcctgtttct aaatttcctg 301 ccgcaattga gctccgccag cttcgaggga tgcccagaag ccaggcttag cggatggatt 361 ccctgaaagc aggaaatggg agccaggctc tctccaggct ctctggggct tagtttgcct 421 tctctggcac ttgaaaggat gaggtcattg ctgtgactga gtggtggaga aaagcagaga 481 aacagagacc aaccactcag ctctgagaaa gcactgggaa agccaaggtt ttaatcaggg 541 gccttaaatc agtcattcat gtttagacgc tgctgagttc ccatggcatg gtttccggat 601 ctaacctcag ttttaaagcc taagtttggc ttgtcagtga gaatgccaag cacacccaag 661 ctttgtaata agagccccga aaaggaaatc ctcgaaaaag gaaacattta aatcatgctt 721 ctcttgggta attttgt //