LOCUS BG070252 836 bp mRNA linear EST 12-MAY-2010
DEFINITION H3085C12-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3085C12 3', mRNA sequence.
ACCESSION BG070252
VERSION BG070252.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 836)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG070252.1.
Other_ESTs: H3085C12-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3085 row: C column: 12
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..836
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3085C12-3"
/db_xref="taxon:10090"
/clone="H3085C12"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 201 a 207 c 181 g 247 t
ORIGIN
1 ctcttcactt tacaaacagc ccctaagcac ttaggacacc acagtatgta tatatgtaac
61 tataaagttc atttccatgc ttattacaga aaaataacta tcagaaatgt cagtctatca
121 ttatcaactg atacacatcc aactcactat agaagggaaa agaaaacaaa acaaaagaaa
181 aaaaaactaa agtaattaaa gcttacaatt ccattttttt tccacttttt ccccctgctt
241 gtttgaactt tttgggtggt ttatctgtgc ttcgctggct gccaggtgat tcccggttaa
301 tggcctccaa gcagattgga gaggaagcct gaggatttct tactttgctg ggcttctgcc
361 ccttggtctt gtgttagtcc cagctcactc tcaatttgat ctagctctgc ctggtcaagt
421 agttcaaagt catccccctc ctcagtgtct gtgtcctctc ccgcagtggg cgccacctga
481 gtgagtgcct gccgggctcc ttctagctgg tctttgatgg cagccgtcat ggcgctgtga
541 tgacatcccc agccaggttg ctcatcaagt gaagggcttg gtcgctcttg agaggaagtg
601 agaggccagc tgccgactgc cgttctttgc ttggtctgtg agcactatcc aactgctgct
661 tctttctctt gagctctgtg ggtaagccca ggcttaattc atcttcatca tttgttcccg
721 tcccattttc cagagatgga aaatctgaaa gatctcgaga gaaaacttcc tcactgggcc
781 ggtcaagatc gtcagaagtg tctgtctgcg gagtgtaccc ctctgaaagg ttgaag
//